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Get e-Alerts Thea 2 toxin Gold nanoparticles with unique optical properties may be useful as biosensors in living whole cells.

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Using a simple and inexpensive technique, we recorded surface plasmon resonance SPR scattering images and SPR absorption spectra from both colloidal gold nanoparticles and from gold nanoparticles conjugated to monoclonal anti-epidermal growth factor receptor anti-EGFR antibodies after incubation in cell cultures with a nonmalignant epithelial cell line HaCaT and two malignant oral epithelial cell lines HOC clone 8 and HSC 3.

Colloidal gold nanoparticles are found in dispersed and aggregated forms within the cell cytoplasm and provide anatomic labeling information, but their uptake is nonspecific for malignant cells.

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This specific and homogeneous binding is found to give a relatively sharper SPR absorption band with a red shifted maximum compared to that observed when added to the noncancerous cells. These results suggest that SPR scattering imaging or SPR absorption spectroscopy generated from antibody conjugated gold nanoparticles can be useful in molecular biosensor techniques for the diagnosis and investigation of oral epithelial living cancer cells in vivo and in vitro.

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Fax: Teri W. Odom Editor-in-Chief. Celebrating 20 years of Nano Letters.

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Nano Letters21 1 Chemistry of Materials32 17 ACS Photonics7 8 Langmuir36 24 The Journal of Physical Chemistry C8 Askins, Mainak Banerjee, Shanta Dhar.

The Journal of Physical Chemistry B5 The Journal of Physical Chemistry C3 ,

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